製品の概要
-
製品名Anti-C3 antibody
C3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to C3
-
特異性This polyclonal antibody detects a band approximately 120 kDa in Western blot under reducing conditions, corresponding to the C3 alpha chain.
-
アプリケーション適用あり: IHC-FoFr, IHC-Fr, WBmore details
-
種交差性交差種: Mouse, Rat
-
免疫原
Unfortunately, this information is considered to be commercially sensitive
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度Protein G purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab11887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-Fr | 1/10. | |
| WB | 1/100. Detects a band of approximately 120 kDa under reducing conditions. |
ターゲット情報
-
機能C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
組織特異性Plasma.
-
関連疾患Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
翻訳後修飾C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在Secreted.
- Information by UniProt
-
参照データベース
- Entrez Gene: 12266 Mouse
- Entrez Gene: 24232 Rat
- SwissProt: P01027 Mouse
- SwissProt: P01026 Rat
- Unigene: 19131 Mouse
- Unigene: 11378 Rat
-
別名
- Acylation stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
see all
Anti-C3 antibody 画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab11887)Positive staining of paraffin embedded mouse kidney tissue with ab11887 at 100x dilution.
-
Immunohistochemistry (Frozen sections) - Anti-C3 antibody (ab11887)Positive staining of frozen mouse spleen tissie with ab11887 at 20x dilution.
-
Immunohistochemistry (Frozen sections) - Anti-C3 antibody (ab11887)Image from Rutar M et al., Journal of Neuroinflammation 2012, 9:257, 26 November 2012. Fig 5.; doi: 10.1186/1742-2094-9-257.Immunohistochemical analysis of rat retina tissue after bright continuous white light exposure, staining C3 with ab11887.
Anti-C3 antibody (ab11887) 使用論文
This product has been referenced in:
- He YY et al. Methyl salicylate 2-O-ß-d-lactoside alleviates the pathological progression of pristane-induced systemic lupus erythematosus-like disease in mice via suppression of inflammatory response and signal transduction. Drug Des Devel Ther 10:3183-3196 (2016). Read more (PubMed: 27729775) »
- Gao TT et al. Complement factors C1q, C3 and C5b-9 in the posterior sclera of guinea pigs with negative lens-defocused myopia. Int J Ophthalmol 8:675-80 (2015). WB ; Guinea pig . Read more (PubMed: 26309860) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"