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C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).
In response to recent customer complaints for IHC-P with paraffin embedded sections we no longer guarantee this application.
Our Abpromise guarantee covers the use of ab11862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml.
Use as coating antibody at 0.5 ug/well in PBS.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/50. Predicted molecular weight: 187 kDa.|
|Flow Cyt||1/50. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Fix tissue sections in acetone.
Immunohistochemical analysis of mouse sciatic nerve section, labelling C3 with ab11862. Mice were intracardially perfused with 4% paraformaldehyde/PBS solution. Following dissection, the sciatic nerve section was embedded and cro-sectioned and immunostained with ab11862 at 1/100.