製品の概要

  • 製品名
    Anti-C3 antibody [11H9]
    C3 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [11H9] to C3
  • 特異性
    This antibody recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. Does not cross react with C4.
  • アプリケーション
    適用あり: ELISA, ICC/IF, WB, Flow Cyt, IHC-Fr, IPmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).

  • 特記事項

    In response to recent customer complaints for IHC-P with paraffin embedded sections we no longer guarantee this application.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.5 µg/ml.

Use as coating antibody at 0.5 ug/well in PBS.

ICC/IF Use at an assay dependent concentration.
WB 1/50. Predicted molecular weight: 187 kDa.
Flow Cyt 1/50.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/50.

Fix tissue sections in acetone.

IP 1/50.

ターゲット情報

  • 機能
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 組織特異性
    Plasma.
  • 関連疾患
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 配列類似性
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • HEL S 62p antibody
    see all

画像

  • Immunohistochemical analysis of mouse sciatic nerve section, labelling C3 with ab11862. Mice were intracardially perfused with 4% paraformaldehyde/PBS solution. Following dissection, the sciatic nerve section was embedded and cro-sectioned and immunostained with ab11862 at 1/100. 

  • ab11862 staining C3 in murine kidney cells by Immunocytochemistry/ Immunofluorescence.
    C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Glomerular staining pattern.

参考文献

This product has been referenced in:
  • Toffoli B  et al. Nephropathy in Pparg-null mice highlights PPAR? systemic activities in metabolism and in the immune system. PLoS One 12:e0171474 (2017). IHC-P ; Mouse . Read more (PubMed: 28182703) »
  • Haure-Mirande JV  et al. Deficiency of TYROBP, an adapter protein for TREM2 and CR3 receptors, is neuroprotective in a mouse model of early Alzheimer's pathology. Acta Neuropathol N/A:N/A (2017). Read more (PubMed: 28612290) »

See all 14 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (ischemia/reperfusion injury)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Permeabilization
No
Specification
ischemia/reperfusion injury
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 14 2016

Application
Western blot
Sample
Mouse Serum (Mouse Serum)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Mouse Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Haoran Zha

Verified customer

投稿 Jan 21 2016

We do not have any IHC data, either in-house or from references, for samples fixed with zinc salts fixatives, and have not received any feedback from customers who have used fixatives other than PFA and formalin. We have no reason to think that this C3...

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Thank you for contacting us.

The antibody has been published in several studies that used it for detection of C3 in mouse samples but not, to our knowledge, in any studies of autoimmune disease or for detection of C3 in human samples.
...

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Thank you for your enquiry and your interest in our products.
I have checked this product (ab19187: Anti-C3 antibody) for you and according to the on-line datasheet it has only been tested in Immunoelectrophoresis.
However, we have an alterna...

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Thank you for your enquiry.

I am sorry to confirm that the C3 antibodies that detect the C3beta subunit have not been tested in WB, human and mouse.

We do have ab11862 Anti-C3 antibody [11H9] which has been tested in WB and human ...

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I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit a...

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Thank you for your enquiry. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, ...

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Thank you for your patience and your e-mail regarding ab11862 monoclonal antibody to mouse C3. As a positive control affinity-purified mouse C3 was used. For the purification of mouse C3 purified antibodies were coupled to CNBr-activated Sepharose 4...

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Thank you for your enquiry. I hope that all is well?The monoclonal antibody 11H9 reacts with C3, C3b, iC3b and C3d. Since C3d is a fragment of the alpha chain we presume the antibody reacts with the alpha chain of C3; however it has not been specifical...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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