製品の概要

  • 製品名
    Anti-C2b antibody
  • 製品の詳細
    Rabbit polyclonal to C2b
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Mouse C2b aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P21180
    (Peptide available as ab95402)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse liver tissue lysate.
  • 特記事項


    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).

ターゲット情報

  • 関連性
    Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • C2 antibody
    • C2b antibody
    • CO2 antibody
    • complement C2 antibody
    • Complement C2b fragment antibody
    • complement component C2 antibody
    see all

画像

  • Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab66999 has not yet been referenced specifically in any publications.

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