The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
Mouse Complement C2 precursor (C2b) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The 97 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C2b.