製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C22orf28
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 107-156 (PEAVVSPGGV GFDINCGVRL LRTNLDESDV QPVKEQLAQA MFDHIPVGVG) of Human C22orf28 (NP_055121).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling C22orf28 with ab98231 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - C22orf28. Right - Merge.


  • Predicted band size : 55 kDa
    Western blot analysis of Jurkat cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling C22orf28 with ab98231 at 1.0µg/ml.


  • Predicted band size : 55 kDa
    Western blot analysis of HeLa cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Anti-C22orf28 antibody (ab98231) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 55 kDa

参考文献

This product has been referenced in:
  • Moore MJ  et al. miRNA-target chimeras reveal miRNA 3'-end pairing as a major determinant of Argonaute target specificity. Nat Commun 6:8864 (2015). WB . Read more (PubMed: 26602609) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
40 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Thank you for your answer and sending the bovine sequence. Indeed, there is a 100% alingment with the immunogen and the chances are therefore very high that the antibody will crossreact. I am thus sending you here the testing discount code. Please see ...

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Thank you very much for your interest in ab98231.

Unfortunately we do not have any trial vials of our products. However if you would like to test this antibody, we can make you an interesting testing offer - please see below.

Do ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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