製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to C22orf28
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QADPNKVSARAKKR

    with a Cysteine residue linker, corresponding to internal sequence amino acids 201-214 of Human C22orf28 (NP_055121.1)

  • ポジティブ・コントロール
    • HeLa, Jurkat and K562 lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab118290 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能
    Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
  • 配列類似性
    Belongs to the RtcB family.
  • 細胞内局在
    Nucleus. Cytoplasm. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ankyrin repeat domain 54 antibody
    • Chromosome 22 open reading frame 28 antibody
    • DJ149A16.6 antibody
    • HSPC117 antibody
    • Hypothetical protein LOC51493 antibody
    • Novel protein HSPC117 antibody
    • OTTHUMP00000028639 antibody
    • RP1-149A16.6 antibody
    • rtcB antibody
    • RTCB_HUMAN antibody
    • tRNA-splicing ligase RtcB homolog antibody
    see all

画像

  • Staining of Human Testis by ab118290 (5μg/mL) in paraffin embedded tissue following stemaed antigen retieval with citrat buffer pH6, AP-Staining this shows nuclear and vicinity staining in spermatogonia.

  • Anti-C22orf28 antibody (ab118290) at 0.3 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

参考文献

ab118290 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
40 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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