製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C22orf25
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 2-51 (CIIFFKFDPR PVSKNAYRLI LAANRDEFYS RPSKLADFWG NNNEILSGLD) of Human C22orf25 (NP_690870).

  • ポジティブ・コントロール
    • Human fetal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-C22orf25 antibody 画像

  • Anti-C22orf25 antibody (ab87576) at 1 µg/ml + human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa

プロトコール

Anti-C22orf25 antibody (ab87576) 使用論文

This product has been referenced in:
  • Lalani SR  et al. Recurrent Muscle Weakness with Rhabdomyolysis, Metabolic Crises, and Cardiac Arrhythmia Due to Bi-allelic TANGO2 Mutations. Am J Hum Genet 98:347-57 (2016). Read more (PubMed: 26805781) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muscle tissue)
Permeabilization
Yes - BSA5%- tween- triton1%
Specification
muscle tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (THYROID GLAND)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CITRATE PH6
Permeabilization
No
Specification
THYROID GLAND
Blocking step
Novolink polymer detection system as blocking agent for 30 minute(s) · Concentration: 0.4% · Temperature: 25°C
Fixative
Formaldehyde
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投稿 May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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