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製品の概要

  • 製品名Anti-C21ORF62 antibody
  • 製品の詳細
    Rabbit polyclonal to C21ORF62
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (STNTLPTEYL AICGLKRLRI NMEAKHPFPE QSLLIHSGGD SDSREKPMWL) of Human C21ORF62 (NP_062542).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • 293T cell lysate. DU145 cell line (IF/ICC)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性The exact function of C21orf62 remains unknown.
  • 参照データベース
    • 別名
      • B37 antibody
      • C21orf120 antibody
      • Chromosome 21 open reading frame 62 antibody
      • Hypothetical protein LOC56245 antibody
      • PRED81 antibody
      • Uncharacterized protein C21orf62 antibody
      see all

    Anti-C21ORF62 antibody 画像

    • Western blot - C21ORF62 antibody (ab84746)
      Western blot - C21ORF62 antibody (ab84746)
      Anti-C21ORF62 antibody (ab84746) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 28 kDa
      Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    • Immunocytochemistry/ Immunofluorescence - Anti-C21ORF62 antibody (ab84746)
      Immunocytochemistry/ Immunofluorescence - Anti-C21ORF62 antibody (ab84746)
      ICC/IF image of ab84746 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-C21ORF62 antibody (ab84746) 使用論文

    ab84746 has not yet been referenced specifically in any publications.

    ab84746 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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