製品の概要
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製品名Anti-C21ORF62 antibody
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製品の詳細Rabbit polyclonal to C21ORF62
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (STNTLPTEYL AICGLKRLRI NMEAKHPFPE QSLLIHSGGD SDSREKPMWL) of Human C21ORF62 (NP_062542).
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ポジティブ･コントロール
- 293T cell lysate. DU145 cell line (IF/ICC)
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab84746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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関連性The exact function of C21orf62 remains unknown.
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参照データベース
- Entrez Gene: 56245 Human
- SwissProt: Q9NYP8 Human
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別名
- B37 antibody
- C21orf120 antibody
- Chromosome 21 open reading frame 62 antibody
see all
画像
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Western blot - C21ORF62 antibody (ab84746)Anti-C21ORF62 antibody (ab84746) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 33 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12% -
Immunocytochemistry/ Immunofluorescence - Anti-C21ORF62 antibody (ab84746)ICC/IF image of ab84746 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献
ab84746 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"