製品の概要

  • 製品名Anti-C20orf19 antibody
    C20orf19 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C20orf19
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 625 and the C-terminus (residue 673) of human C20orf19 (NP_060944.2)

  • ポジティブ・コントロール
    • Whole cell lysates from Hela and 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 75 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
  • 配列類似性Belongs to the kizuna family.
  • 翻訳後修飾Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome. Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Centrosomal protein kizuna antibody
    • DKFZP586H021 antibody
    • HT013 antibody
    • Kiz antibody
    • KIZ_HUMAN antibody
    • KIZUNA antibody
    • MGC102941 antibody
    • MGC141930 antibody
    • NCRNA00153 antibody
    • Plk1s1 antibody
    • Polo-like kinase 1 substrate 1 antibody
    • Uncharacterized protein C20orf19 antibody
    see all

Anti-C20orf19 antibody 画像

  • All lanes : Anti-C20orf19 antibody (ab72248) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 75 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Detection of C20orf19 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72248 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-C20orf19 antibody (ab72248) 使用論文

ab72248 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72248.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"