製品の概要

  • 製品名
    Anti-C2 antibody [6E3]
    C2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6E3] to C2
  • 由来種
    Mouse
  • 特異性
    Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2. ab17452 reacts with C2 and a subfraction of C2 believed to be C2b.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native complement component C2 protein, isolated from human plasma.

  • エピトープ
    Epitope specificity differs from that of ab17451 as determined by inhibition ELISA.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    6E3
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/500.
    WB: 1/250. Predicted molecular weight: 102 kDa. ab17452 reacts with C2 under reducing and non-reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
    • 関連疾患
      Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
    • 配列類似性
      Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
      Contains 3 Sushi (CCP/SCR) domains.
      Contains 1 VWFA domain.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
      • 別名
        • ARMD14 antibody
        • C2 antibody
        • C3/C5 convertase antibody
        • CO2 antibody
        • CO2_HUMAN antibody
        • Complement C2 antibody
        • Complement C2a fragment antibody
        • complement component 2 antibody
        • DKFZp779M0311 antibody
        see all

      プロトコール

      参考文献

      This product has been referenced in:
      • Martini PG  et al. Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases. BMC Immunol 11:43 (2010). Sandwich ELISA ; Human . Read more (PubMed: 20727163) »

      See 1 Publication for this product

      ab17452 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      1 Review or Q&A

      10
      Votes: Highest First

      What was the ELISA protocol that was used to test these antibodies?

      Thank you for your enquiry. I received the following information from the originator of these two antibodies: "The protocol we used to test these antibodies was a direct ELISA with a coat of C2, a layer with ab17451 (1:6500) or ab17452 (1:500), and det...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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