製品の概要

  • 製品名
    Anti-C2 antibody [5C3]
    C2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5C3] to C2
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with C2 and a subfraction of C2 believed to be C2b
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native complement component C2 protein isolated from human plasma.

  • エピトープ
    Epitope specificity differs from that of ab17452 as determined by inhibition ELISA.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    5C3
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/12000.
WB Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.

ターゲット情報

  • 機能
    Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • 関連疾患
    Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ARMD14 antibody
      • C2 antibody
      • C3/C5 convertase antibody
      • CO2 antibody
      • CO2_HUMAN antibody
      • Complement C2 antibody
      • Complement C2a fragment antibody
      • complement component 2 antibody
      • DKFZp779M0311 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Rorbach J  et al. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21666256) »
    • Stenbaek EI  et al. Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-86 (1986). Read more (PubMed: 3796629) »

    See all 2 Publications for this product

    ab17451 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

    10
    Votes: Highest First

    Hola.
    Tengo una duda acerca de la especificidad de este anticuerpo, querría saber cual es su especificidad y si reconoce únicamentealgún fragmento del C2 (C2a o C2b)o el C2 en su totalidad.
    Muchas gracias

    Gracias por contactarnos. Te pido disculpas por la espera.

    ab17451 reacciona con la proteína C2 humana en sus formas nativa y desnaturalizada. En WB el anticuerpo reconoce C2 y una fracción de la misma, probablemente C2b, ambas ...

    Read More

    What was the ELISA protocol that was used to test these antibodies?

    Thank you for your enquiry. I received the following information from the originator of these two antibodies: "The protocol we used to test these antibodies was a direct ELISA with a coat of C2, a layer with ab17451 (1:6500) or ab17452 (1:500), and det...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録