製品の概要

  • 製品名Anti-C1s antibody
    C1s 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C1s
  • 特異性ab72651 detects endogenous levels of fragment of activated C1s resulting from cleavage adjacent to Arg437.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from human C1s.

  • ポジティブ・コントロール
    • Extracts from 293 cells treated with etoposide (25uM, 1hour) and HeLa cells treated with etoposide (25uM, 24hours).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects bands of approximately 75 & 51 kDa (predicted molecular weight: 77 & 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
    • 関連疾患Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • 配列類似性Belongs to the peptidase S1 family.
      Contains 2 CUB domains.
      Contains 1 EGF-like domain.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • 翻訳後修飾The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Basic proline rich peptide IB 1 antibody
      • C1 esterase antibody
      • C1S antibody
      • C1S_HUMAN antibody
      • Complement C1s subcomponent antibody
      • Complement C1s subcomponent heavy chain antibody
      • Complement C1s subcomponent light chain antibody
      • Complement component 1 s subcomponent antibody
      • Complement component 1 subcomponent s antibody
      • FLJ44757 antibody
      see all

    Anti-C1s antibody 画像

    • All lanes : Anti-C1s antibody (ab72651) at 1/500 dilution

      Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
      Lane 2 : Extracts from HeLa cells treated with etoposide (25uM, 24hours)
      Lane 3 : Extracts from HeLa cells treated with etoposide (25uM, 24hours) with Cl-peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 77 , 51 kDa
      Observed band size : 51,75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Anti-C1s antibody (ab72651) 使用論文

    ab72651 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72651.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"