製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C1r
  • 特異性
    ab72648 detects a fragment (light chain) of activated C1r resulting from cleavage adjacent to Ile464.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from human C1r.

  • ポジティブ・コントロール
    • Extracts from 293 cells treated with etoposide (25uM, 1hour) and A549 cells treated with etoposide (25uM, 24hours).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能
    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 翻訳後修飾
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1 R antibody
    • C1R antibody
    • C1R_HUMAN antibody
    • Complement C1r antibody
    • Complement C1r subcomponent antibody
    • Complement C1r subcomponent light chain antibody
    • Complement C1r subcomponent precursor antibody
    • Complement component 1 r subcomponent antibody
    • Complement component 1 subcomponent r antibody
    see all

画像

  • All lanes : Anti-C1r antibody (ab72648) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from A549 cells treated with etoposide (25uM, 24hours)
    Lane 3 : Extracts from 293 cells treated with etoposide (25uM, 1hour) with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 19 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab72648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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