Rabbit polyclonal to C1r
Non human primates
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human C1r.
(Peptide available as
This antibody gave a positive signal in Human plasma (total protein)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
Belongs to the peptidase S1 family.
Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Information by UniProt
C1 R antibody
Western blot - Anti-C1r antibody (ab66751)
All lanes :
Anti-C1r antibody (ab66751) at 1 µg/ml
Lane 1 :
Human Plasma Total Protein Lysate at 2 µg
Lane 2 :
Human Plasma Total Protein Lysate at 5 µg
Lane 3 :
Human Plasma Total Protein Lysate at 10 µg
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
Performed under reducing conditions.
Predicted band size:
Observed band size:
90 kDa (
why is the actual band size different from the predicted?
C1r contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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