Anti-C1QC 抗体 [EPR2984Y] (ab75756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2984Y] to C1QC
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-C1QC antibody [EPR2984Y]
C1QC 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2984Y] to C1QC -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human C1QC aa 150-250. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human plasma lysate; IHC-P: Human liver tissue; Flow Cyt (intra): Human Peripheral Blood Mononuclear Cell (PBMC) cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2984Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75756の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
関連疾患
Defects in C1QC are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 714 Human
- Omim: 120575 Human
- SwissProt: P02747 Human
- Unigene: 467753 Human
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別名
- AI385742 antibody
- C1Q C antibody
- C1qc antibody
see all
画像
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ab75756 at 1/100 dilution staining C1q in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-C1QC antibody [EPR2984Y] (ab75756) at 1/1000 dilution + human plasma lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
Flow Cytometry analysis of Human Peripheral Blood Mononuclear Cell (PBMC) cells labeling C1QC with purified ab75756 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 2% Paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Left).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab75756 は 4 報の論文で使用されています。
- Zhai M et al. Identification of Three Significant Genes Associated with Immune Cells Infiltration in Dysfunctional Adipose Tissue-Induced Insulin-Resistance of Obese Patients via Comprehensive Bioinformatics Analysis. Int J Endocrinol 2021:8820089 (2021). PubMed: 33564304
- Kim EN et al. CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm. PLoS One 16:e0245361 (2021). PubMed: 34428207
- Wolf C et al. Janus kinase inhibition in complement component 1 deficiency. J Allergy Clin Immunol 146:1439-1442.e5 (2020). PubMed: 32325142
- Heo SH et al. Fabry disease: characterisation of the plasma proteome pre- and post-enzyme replacement therapy. J Med Genet 54:771-780 (2017). PubMed: 28835480