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Our Abpromise guarantee covers the use of ab90181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab90181 staining C1QB in Rat brain tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Tween 29 and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/600 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody.
ab90181 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"