製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C1QA
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse C1QA aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91203)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse blood plasma tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).

ターゲット情報

  • 機能
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • 関連疾患
    Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • 配列類似性
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • 翻訳後修飾
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1qa antibody
    • C1QA_HUMAN antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement component 1 q subcomponent A chain antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    see all

Anti-C1QA antibody 画像

  • Anti-C1QA antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 36 + 33 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutesThe 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor. C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

プロトコール

Anti-C1QA antibody (ab64632) 使用論文

ab64632 has not yet been referenced specifically in any publications.

Product Wall


The C1q antibody ab64632 is specific for subunit A.

Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (Brain tissue lysate)
Specification
Brain tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Dec 24 2013

Thank you for contacting us. None of our antibodies have been validated for in vivo or diagnostic use. This C1q antibody in particular (ab64632) contains sodium azide, which can be very toxic in vivo. I hope this helps, please let me kno...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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