製品の概要

  • 製品名
    Anti-C1q antibody [9A7]
    C1q 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9A7] to C1q
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    C1q protein purified from Human plasma

  • ポジティブ・コントロール
    • K562, Jurkat, C6 and NIH3T3 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-C1q antibody [9A7] 画像

  • All lanes : Anti-C1q antibody [9A7] (ab71089) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat T cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab71089 (1µg/ml) staining C1q in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of of the epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-C1q antibody [9A7] (ab71089) 使用論文

This product has been referenced in:
  • Lui H  et al. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell 165:921-35 (2016). Flow Cyt ; Mouse . Read more (PubMed: 27114033) »
  • Astafurov K  et al. Complement expression in the retina is not influenced by short-term pressure elevation. Mol Vis 20:140-52 (2014). WB . Read more (PubMed: 24505213) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Human Serum (Serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Serum
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Username

任弘 東岸

Verified customer

投稿 Aug 06 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana - cc1mild
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Thermo SuperBlock Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Dr. Lampros Panagis

Verified customer

投稿 Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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