製品の概要

  • 製品名Anti-C1q antibody [9A7]
    C1q 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9A7] to C1q
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    C1q protein purified from Human plasma

  • ポジティブ・コントロール
    • K562, Jurkat, C6 and NIH3T3 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-C1q antibody [9A7] 画像

  • All lanes : Anti-C1q antibody [9A7] (ab71089) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat T cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab71089 (1µg/ml) staining C1q in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of of the epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-C1q antibody [9A7] (ab71089) 使用論文

This product has been referenced in:
  • Astafurov K  et al. Complement expression in the retina is not influenced by short-term pressure elevation. Mol Vis 20:140-52 (2014). WB . Read more (PubMed: 24505213) »
  • Adzemovic MZ  et al. Efficacy of vitamin D in treating multiple sclerosis-like neuroinflammation depends on developmental stage. Exp Neurol 249:39-48 (2013). Read more (PubMed: 23954214) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Serum (Serum)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Serum
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Username

任弘 東岸

Verified customer

投稿 Aug 06 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana - cc1mild
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Brain)
Loading amount 20 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Thermo SuperBlock Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Dr. Lampros Panagis

Verified customer

投稿 Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"