製品の概要

  • 製品名
    Anti-C1q antibody [7H8]
    C1q 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [7H8] to C1q
  • 特異性
    Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.
  • アプリケーション
    適用あり: ICC, IHC-FoFr, ICC/IF, ELISA, Functional Studies, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    This information is considered to be commercially sensitive

  • ポジティブ・コントロール
    • C1q can be found on macrophages, follicular dendritic cells, interdigitating cells and further cells of the monocyte-macrophage lineage. These types of cells can be used as positive control.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    0.2 µm filtered
  • ポリ/モノ
    モノクローナル
  • クローン名
    7H8
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/10.
IHC-FoFr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.
IHC-Fr 1/10.

ターゲット情報

  • 機能
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • 関連疾患
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • 配列類似性
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • 翻訳後修飾
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

画像

  • ab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.

    See Abreview

参考文献

This product has been referenced in:
  • Zhang Z  et al. Dysregulation of synaptogenesis genes antecedes motor neuron pathology in spinal muscular atrophy. Proc Natl Acad Sci U S A 110:19348-53 (2013). ICC/IF ; Mouse . Read more (PubMed: 24191055) »
  • Linnartz B  et al. Sialic acid on the neuronal glycocalyx prevents complement C1 binding and complement receptor-3-mediated removal by microglia. J Neurosci 32:946-52 (2012). ICC/IF ; Mouse . Read more (PubMed: 22262892) »

See all 8 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Haoran Zha

Verified customer

投稿 Mar 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (Neuro2a cell line)
Specification
Neuro2a cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Aug 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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