アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
This information is considered to be commercially sensitive
Our Abpromise guarantee covers the use of ab11861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent dilution.|
|Functional Studies||Use at an assay dependent dilution.|
ab11861 staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 1% paraformaldehyde and blocked with 10% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS) for 2 hours at 24°C. An undiluted Alexa Fluor®488-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature.