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antigen sequence: EVRRYIMEKI VQANKLLQNQ EPVNDKRERK LKFKDQLVDL EVPPLEDTTT SKNYFENERN MFGKLSQLCI SNDFGQEDVL LSLTNGSCE, corresponding to amino acids 122-210 of Human C1orf114 (Q5TID7).
Our Abpromise guarantee covers the use of ab121924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.|
ab121924 has not yet been referenced specifically in any publications.