製品の概要

  • 製品名Anti-C1orf106 antibody
    C1orf106 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C1orf106
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: PPQTLEGLQP TGPEAGSPER APVQNSPWKE TSLDHPYEKP RKSSEPWSES SSPATTPQDG PSASSLWLLE PASYHVVPIR G, corresponding to amino acids 297 - 377 of Human C1orf106 (UniProt ID: Q3KP66).

  • ポジティブ・コントロール
    • Human stomach tissue
      RT-4 lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/500 - 1/1000.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-C1orf106 antibody 画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121945 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C1orf106 antibody (ab121945) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab121945, at a 1/85 dilution, staining C1orf106 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Anti-C1orf106 antibody (ab121945) 使用論文

ab121945 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"