製品の概要
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製品名Anti-C1GALT1C1 antibody
C1GALT1C1 一次抗体 製品一覧 -
製品の詳細Mouse polyclonal to C1GALT1C1
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由来種Mouse
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特異性This antibody is reactive against mammalian transfected lysate.
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アプリケーション適用あり: WB, ICC/IFmore details
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種交差性交差種: Human
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免疫原
C1GALT1C1 (NP_001011551.1, 1 a.a. ~ 318 a.a) full-length human protein.
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ポジティブ･コントロール
- C1GALT1C1 transfected 293T cell lysate an human liver tissue lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
精製度Protein G purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab68310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa). | |
| ICC/IF | 1/100. |
ターゲット情報
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機能Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
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組織特異性Ubiquitously expressed. Abundantly expressed in salivary gland, stomach, small intestine, kidney, and testis and at intermediate levels in whole brain, cerebellum, spinal cord, thymus, spleen, trachea, lung, pancreas, ovary, and uterus.
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関連疾患Defects in C1GALT1C1 are the cause of Tn syndrome (TNSYN) [MIM:300622]. Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state.
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配列類似性Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 29071 Human
- Omim: 300611 Human
- SwissProt: Q96EU7 Human
- Unigene: 643920 Human
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別名
- HSPC067 antibody
- 3-galactosyltransferase 2 antibody
- Beta 1,3 galactosyltransferase 2 antibody
see all
画像
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Western blot - C1GALT1C1 antibody (ab68310)Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 36 kDa
Observed band size: 32 kDa (why is the actual band size different from the predicted?) -
Western blot - C1GALT1C1 antibody (ab68310)All lanes : Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution
Lane 1 : C1GALT1C1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 36 kDa
Observed band size: 32 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-C1GALT1C1 antibody (ab68310)This image is courtesy of an Abreview submitted by Armen Petrosyanab68310 staining C1GALT1C1 in the Human Panc-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% Donkey serum) for 1 hour at 22°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody.
Key Green-C1GALT1C1, Red-ab24586 Giantin-Golgi marker, Blue-Nucleus (DAPI)
参考文献
ab68310 has not yet been referenced specifically in any publications.
レビューと Q&A
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