製品の概要

  • 製品名Anti-C1GALT1C1 antibody
    C1GALT1C1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to C1GALT1C1
  • 特異性This antibody is reactive against mammalian transfected lysate.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    C1GALT1C1 (NP_001011551.1, 1 a.a. ~ 318 a.a) full-length human protein.

  • ポジティブ・コントロール
    • C1GALT1C1 transfected 293T cell lysate an human liver tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100.

ターゲット情報

  • 機能Probable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
  • 組織特異性Ubiquitously expressed. Abundantly expressed in salivary gland, stomach, small intestine, kidney, and testis and at intermediate levels in whole brain, cerebellum, spinal cord, thymus, spleen, trachea, lung, pancreas, ovary, and uterus.
  • 関連疾患Defects in C1GALT1C1 are the cause of Tn syndrome (TNSYN) [MIM:300622]. Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state.
  • 配列類似性Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSPC067 antibody
    • 3-galactosyltransferase 2 antibody
    • Beta 1,3 galactosyltransferase 2 antibody
    • Beta1,3 galactosyltransferase 2 antibody
    • C1Gal T2 antibody
    • C1Gal-T2 antibody
    • C1GALT1 specific chaperone 1 antibody
    • C1GALT1-specific chaperone 1 antibody
    • C1galt1c1 antibody
    • C1GalT2 antibody
    • C1GLC_HUMAN antibody
    • C38H2 L1 antibody
    • C38H2 like protein 1 antibody
    • C38H2-L1 antibody
    • C38H2-like protein 1 antibody
    • C38H2L1 antibody
    • Core 1 beta1 antibody
    • Core 1 beta3 galactosyltransferase specific molecular chaperone antibody
    • Core 1 beta3-Gal-T2 antibody
    • Core 1 beta3-galactosyltransferase-specific molecular chaperone antibody
    • Core 1 UDP galactose:N acetylgalactosamine alpha R beta 1,3 galactosyltransferase 2 antibody
    • COSMC antibody
    • MGC19947 antibody
    • MST143 antibody
    see all

Anti-C1GALT1C1 antibody 画像

  • Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-C1GALT1C1 antibody (ab68310) at 1/500 dilution

    Lane 1 : C1GALT1C1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 36 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab68310 staining C1GALT1C1 in the Human Panc-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% Donkey serum) for 1 hour at 22°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody.
    Key Green-C1GALT1C1, Red-ab24586 Giantin-Golgi marker, Blue-Nucleus (DAPI)

    See Abreview

Anti-C1GALT1C1 antibody (ab68310) 使用論文

ab68310 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"