The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5-10 µg/mg of lysate.
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
Not suitable for crude preparations (e.g. whole cell lysate). Can be used for WB of enriched (e.g. immunoprecipitated) sources of C19orf61.
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited by release factors to stalled ribosomes together with SMG1 and SMG8 (forming the SMG1C protein kinase complex) and, in the SMG1C complex, is required for the efficient association between SMG1 and SMG8.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling C19orf61 with ab85689 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - C19orf61 antibody (ab85659)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg ab90659. 20% of the immunoprecipitate was loaded in each lane, and probed with ab90659 at 1µg/ml (lane 1) or with a control IgG (lane 2). Detection: chemoluminescence, with an exposure time of 1 second.
Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol19:4 (2018).
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