製品の概要

  • 製品名Anti-C19orf21 antibody
    C19orf21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C19orf21
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 578-627 (PTPDENSDQN SRSSSQASGI TGSYSVSESP FFSPIHLHSN VAWTVEDPVD) of Human C19orf21 (NP_775752).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.
  • 発生段階Regulated in a cell-cycle dependent manner. Weakly expressed in G1 and S phases. Expression increases in G2/M phases and persisting until the end of mitosis (at protein level).
  • 翻訳後修飾Phosphorylated by CDK1 and PLK1. CDK1 is the priming kinase for PLK1 phosphorylation. Phosphorylation by PLK1 is required for proper spindle orientation at metaphase.
  • 細胞内局在Cell junction > focal adhesion. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Predominantly localizes to cortical actin structures during interphase and mitosis. Present in retraction fibers, which are formed at former adhesion sites during mitosis, and at spicular membrane protrusions in re-attaching cytokinetic cells. Partially colocalizes with cytoplasmic F-actin. Not detected at microtubules at interphase, nor at spindle during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • chromosome 19 open reading frame 21 antibody
    • hypothetical protein LOC126353 antibody
    • Misp antibody
    • MISP_HUMAN antibody
    • Mitotic interactor and substrate of PLK1 antibody
    • Mitotic spindle positioning protein antibody
    • Uncharacterized protein C19orf21 antibody
    see all

Anti-C19orf21 antibody 画像

  • Anti-C19orf21 antibody (ab81302) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

Anti-C19orf21 antibody (ab81302) 使用論文

ab81302 has not yet been referenced specifically in any publications.

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