製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C19orf2
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and 400 of human C19orf2, BAA34781.1.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival sigaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity at mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.
  • 組織特異性
    Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.
  • 配列類似性
    Belongs to the RNA polymerase II subunit 5-mediating protein family.
  • 翻訳後修飾
    Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, releaves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity and preventing sustained S6K1-dependent signaling.
  • 細胞内局在
    Nucleus. Cytoplasm. Mitochondrion. Cell projection > dendrite. Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C19orf2 antibody
    • Chromosome 19 open reading frame 2 antibody
    • NNX3 antibody
    • NNX3 protein antibody
    • PPP1R19 antibody
    • Protein NNX3 antibody
    • Protein phosphatase 1 regulatory subunit 19 antibody
    • RMP antibody
    • RMP_HUMAN antibody
    • RNA polymerase II subunit 5 mediating protein antibody
    • RNA polymerase II subunit 5-mediating protein antibody
    • RPB5 mediating protein antibody
    • RPB5-mediating protein antibody
    • Unconventional prefoldin RPB5 interactor 1 antibody
    • Unconventional prefoldin RPB5 interactor antibody
    • URI antibody
    • URI1 antibody
    • URI1, prefoldin-like chaperone antibody
    see all

画像

  • All lanes : Anti-C19orf2 antibody (ab72134) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of C19orf2.
    Lane 1: ab72134 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72134 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 10 seconds.

参考文献

ab72134 has not yet been referenced specifically in any publications.

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