製品の概要

  • 製品名Anti-C17orf71 antibody
    C17orf71 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C17orf71
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding a region between residues 250 and 300 of human C17orf71 (NP_060619.4)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibody was affinity purified using an epitope specific to C17orf71 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 関連性C17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.
    • 参照データベース
    • 別名
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      • FLJ10587 antibody
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      • protein SMG8 antibody
      • SMG8 antibody
      • SMG8 protein antibody
      see all

    Anti-C17orf71 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling C17orf71 with ab80472 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of human C17orf71 by Western Blot of Immunoprecipitates in whole cell lysates (1 mg for IP, 20% of IP loaded) from HeLa cells (lane 1), using 3 µg/mg of lysate of ab80472 for IP.
      Lane 2: Control IgG.
      For blotting immunoprecipitated C17orf71, ab80472 was used at 1 µg/ml.
      Detection: via Chemiluminescence with an exposure time of 10 seconds.

    Anti-C17orf71 antibody (ab80472) 使用論文

    ab80472 has not yet been referenced specifically in any publications.

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