製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C17orf71
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • 免疫原

    Synthetic peptide corresponding a region between residues 250 and 300 of human C17orf71 (NP_060619.4)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibody was affinity purified using an epitope specific to C17orf71 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 関連性
      C17orf71 belongs to the UPF0487 family. There are 3 isoforms produced by alternative splicing.
    • 参照データベース
    • 別名
      • ABC 2 antibody
      • ABC2 antibody
      • Amplified in breast cancer gene 2 protein antibody
      • Chromosome 17 open reading antibody
      • chromosome 17 open reading frame 71 antibody
      • FLJ10587 antibody
      • Protein smg 8 homolog antibody
      • protein SMG8 antibody
      • SMG8 antibody
      • SMG8 protein antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling C17orf71 with ab80472 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of human C17orf71 by Western Blot of Immunoprecipitates in whole cell lysates (1 mg for IP, 20% of IP loaded) from HeLa cells (lane 1), using 3 µg/mg of lysate of ab80472 for IP.
      Lane 2: Control IgG.
      For blotting immunoprecipitated C17orf71, ab80472 was used at 1 µg/ml.
      Detection: via Chemiluminescence with an exposure time of 10 seconds.

    参考文献

    ab80472 has not yet been referenced specifically in any publications.

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