製品の概要

  • 製品名
    Anti-C17orf53 antibody
  • 製品の詳細
    Rabbit polyclonal to C17orf53
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 587-617 of Human C17orf53 (NP_001164722.1, NP_076937.2)

  • ポジティブ・コントロール
    • CEM cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 70 kDa.

ターゲット情報

  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 3 isoforms produced by alternative splicing. Immunogen absent in isoforms 2 and 3.
  • 別名
    • C17orf53 antibody
    • Chromosome 17 open reading frame 53 antibody
    • CQ053_HUMAN antibody
    • FLJ11594 antibody
    • Hypothetical protein LOC78995 antibody
    • MGC3130 antibody
    • Uncharacterized protein C17orf53 antibody
    see all

画像

  • Anti-C17orf53 antibody (ab107354) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size : 70 kDa

プロトコール

参考文献

ab107354 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Kyoto)
Loading amount
35 µg
Specification
HeLa Kyoto
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 13 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Kyoto)
Specification
HeLa Kyoto
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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