製品の概要

  • 製品名Anti-C15orf42 antibody
    C15orf42 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C15orf42
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 975-1025 of Human C15orf42 (NP_689472.3).

  • ポジティブ・コントロール
    • 293T whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab124268 was affinity purified using an epitope specific to C15orf42 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab124268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2500. Predicted molecular weight: 211 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating to CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation.
    • 配列類似性Belongs to the treslin family.
    • 細胞内局在Nucleus. Associates with chromatin.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C15orf42 antibody
      • chromosome 15 open reading frame 42 antibody
      • replication-stimulating protein antibody
      • SLD3 antibody
      • SLD3 homolog antibody
      • SLD3, yeast , homolog of antibody
      • TICRR antibody
      • TICRR_HUMAN antibody
      • TopBP1-interacting antibody
      • TopBP1-interacting checkpoint and replication regulator antibody
      • Treslin antibody
      see all

    Anti-C15orf42 antibody 画像

    • All lanes : Anti-C15orf42 antibody (ab124268) at 1/1000 dilution

      Lane 1 : 293 whole cell lysate treated with control siLUC RNAi
      Lane 2 : 293 whole cell lysate treated with RNAi targeting C15orf42
      Lane 3 : 293 whole cell lysate treated with RNAi targeting C15orf42

      Lysates/proteins at 50 µg per lane.

      Secondary
      Donkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/20000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 211 kDa
      Observed band size : 250 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa (possible non-specific binding).

      Exposure time : 2 minutes

      This image is courtesy of an anonymous abreview.

      Blocking: 5% milk

    • All lanes : Anti-C15orf42 antibody (ab124268) at 0.4 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg

      Developed using the ECL technique

      Predicted band size : 211 kDa


      Exposure time : 3 minutes

    Anti-C15orf42 antibody (ab124268) 使用論文

    ab124268 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (7.5%)
    Sample Human Cell lysate - whole cell (HEK293 cells)
    Specification HEK293 cells
    Treatment siRNA for 72h
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

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    投稿 May 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"