製品の概要

  • 製品名Anti-C14orf166 antibody
    C14orf166 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C14orf166
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Dog
  • 免疫原

    A region within synthetic peptide: GLAVRLEYGD NAEKYKDLVP DNSKTADNAT KNAEPLINLD VNNPDFKAGV, corresponding to amino acids 85-134 of Human C14orf166

  • ポジティブ・コントロール
    • Jurkat cell lysate. IF/ICC: HeLa cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P 1/100.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 組織特異性Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors.
  • 配列類似性Belongs to the UPF0568 family.
  • 細胞内局在Nucleus. Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. May localize at the centrosome during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C14orf166 antibody
    • CGI 99 antibody
    • CGI-99 antibody
    • CGI99 antibody
    • Chromosome 14 open reading frame 166 antibody
    • CLE antibody
    • CLE7 antibody
    • CN166_HUMAN antibody
    • Homeobox prox 1 antibody
    • LCRP369 antibody
    • RLL motif containing 1 antibody
    • RLLM1 antibody
    • UPF0568 protein C14orf166 antibody
    see all

Anti-C14orf166 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling C14orf166 with ab49342 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown as cytoplasmic. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - C14orf166. Right - Merge.
  • ICC/IF image of ab49342 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49342, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-C14orf166 antibody (ab49342) at 0.125 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : ~26 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

Anti-C14orf166 antibody (ab49342) 使用論文

This product has been referenced in:
  • Pérez-González A  et al. hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PLoS One 9:e90957 (2014). WB ; Human . Read more (PubMed: 24608264) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"