製品の概要

  • 製品名Anti-C14orf130 antibody
    C14orf130 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C14orf130
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: MIQCVVCEDW FHGRHLGAIP PESGDFQEMV CQACMKRCSF LWAYAAQLAV, corresponding to internal sequence amino acids 152-201 of Human C14orf130

  • ポジティブ・コントロール
    • Jurkat cell lysate and human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性C14orf130 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
  • 参照データベース
  • 別名
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    • FLJ10483 antibody
    • MGC94098 antibody
    • MGC9518 antibody
    • N recognin 7 antibody
    • Putative E3 ubiquitin-protein ligase UBR7 antibody
    • RGD1359144 antibody
    • Ubiquitin protein ligase E3 component n-recognin 7 (putative) antibody
    • UBR7 antibody
    see all

Anti-C14orf130 antibody 画像

  • Anti-C14orf130 antibody (ab59151) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C14orf130 with ab59151 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-C14orf130 antibody (ab59151) 使用論文

ab59151 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"