Anti-C14orf106 抗体 (ab89265)
Key features and details
- Mouse polyclonal to C14orf106
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-C14orf106 antibody -
製品の詳細
Mouse polyclonal to C14orf106 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human C14orf106. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: Human spleen tissue lysate, C14orf106 transfected 293T cell lysate ICC/IF: HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
ICC/IF |
特記事項 |
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WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa for isoform 1 and 44 kDa for isoform 2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
C14orf106 is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It interacts with SP1. There are two isoforms. -
細胞内局在
Nucleus. Centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. -
参照データベース
- Entrez Gene: 55320 Human
- SwissProt: Q6P0N0 Human
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別名
- Chromosome 14 open reading frame 106 antibody
- HsKNL-2 antibody
- Kinetochore-associated protein KNL-2 homolog antibody
see all
画像
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Anti-C14orf106 antibody (ab89265) at 1 µg/ml + human spleen tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 129 , 44 kDa
Observed band size: ~129 kDa why is the actual band size different from the predicted? -
All lanes : Anti-C14orf106 antibody (ab89265) at 1 µg/ml
Lane 1 : C14orf106 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 , 44 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
ab89265, at 10 µg/ml, staining C14orf106 in HeLa cell by Immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab89265 は論文での使用が確認できていません。