製品の概要

  • 製品名
    Anti-C14orf106 antibody
  • 製品の詳細
    Mouse polyclonal to C14orf106
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human C14orf106 protein (AAH51885.1; or aa 1-314 of Q6P0N0-1 and Q6P0N0-2).

  • ポジティブ・コントロール
    • WB: Human spleen tissue lysate, C14orf106 transfected 293T cell lysate ICC/IF: HeLa cell

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa for isoform 1 and 44 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      C14orf106 is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It interacts with SP1. There are two isoforms.
    • 細胞内局在
      Nucleus. Centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.
    • 参照データベース
    • 別名
      • Chromosome 14 open reading frame 106 antibody
      • HsKNL-2 antibody
      • Kinetochore-associated protein KNL-2 homolog antibody
      • M18BP1 antibody
      • Mis18-binding protein 1 antibody
      • MIS18BP1 antibody
      • Putative protein p243 which interacts with transcription factor Sp1 antibody
      • Uncharacterized potential DNA binding protein C14orf106 antibody
      see all

    画像

    • Anti-C14orf106 antibody (ab89265) at 1 µg/ml + human spleen tissue lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 129 , 44 kDa
      Observed band size : ~129 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-C14orf106 antibody (ab89265) at 1 µg/ml

      Lane 1 : C14orf106 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Developed using the ECL technique

      Predicted band size : 129 , 44 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • ab89265, at 10 µg/ml, staining C14orf106 in HeLa cell by Immunofluorescence.

    参考文献

    ab89265 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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