All lanes : Anti-C13orf7 antibody (ab91464) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 81 kDa
Immunoprecipitation - C13orf7 antibody (ab91464)
Sample: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) . Antibody: ab91464 at 1 µg/ml for WB and at 10 µg/mg lysate for IP. Detection: chemiluminescence with exposure times of 10 seconds.