The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
機能May act as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion.
関連疾患Defects in C12orf65 are the cause of combined oxidative phosphorylation deficiency type 7 (COXPD7) [MIM:613559]. A mitochondrial disease resulting in encephalomyopathy. Clinical manifestations include psychomotor delay and regression, ataxia, optic atrophy, nystagmus and muscle atrophy and weakness.
配列類似性Belongs to the prokaryotic/mitochondrial release factor family.