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製品の概要

  • 製品名Anti-C12orf48 antibody - C-terminal
    C12orf48 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C12orf48 - C-terminal
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 529-558 from the C terminal region of Human C12orf48 (NP_060385.3).

  • ポジティブ･コントロール
    • A549 cell line lysate. This antibody gave a positive result when used in the following methanol fixed cell line: HeLa.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項（精製）ab107308 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG

アプリケーション

Our Abpromise guarantee covers the use of ab107308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 65 kDa.

ターゲット情報

  • 機能May be involved in RNA metabolism. Binds single-strand DNA and poly(A) homopolymers (By similarity). Positively regulate the poly(ADP-ribosyl)ation activity of PARP1.
  • 組織特異性Restricted to testis. Overexpressed in multiple cancer cells.
  • 配列類似性Belongs to the PARP1BP family.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • AROM antibody
      • C12orf48 antibody
      • Chromosome 12 open reading frame 48 antibody
      • FLJ20641 antibody
      • PARP 1 binding protein antibody
      • PARP-1 binding protein antibody
      • PARP1 binding protein antibody
      • PARP1-binding protein antibody
      • PARPBP antibody
      • PR1BP_HUMAN antibody
      see all

    Anti-C12orf48 antibody - C-terminal 画像

    • Immunocytochemistry/ Immunofluorescence - Anti-C12orf48 antibody - C-terminal (ab107308)
      Immunocytochemistry/ Immunofluorescence - Anti-C12orf48 antibody - C-terminal (ab107308)

      ICC/IF image of ab107308 stained HeLa cells.  The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107308 at 5µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-C12orf48 antibody - C-terminal (ab107308)
      Western blot - Anti-C12orf48 antibody - C-terminal (ab107308)
      Anti-C12orf48 antibody - C-terminal (ab107308) at 1/100 dilution + A549 cell line lysate at 35 µg

      Predicted band size : 65 kDa

    Anti-C12orf48 antibody - C-terminal (ab107308) 使用論文

    This product has been referenced in:
    • Hampp S  et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . Read more (PubMed: 27407148) »

    See 1 Publication for this product

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