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antigen sequence: VIVPTSSSGQHRQRPALGGAGTLEGVEASLFYQCLENLCDRHKYSCPPPA LVKEALSNVQ RLTFYGFLMALSKHRGINQAL, corresponding to amino acids 172-252 of Human C11orf49 (Q9H6J7).
Our Abpromise guarantee covers the use of ab122646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.|
ab122646 has not yet been referenced specifically in any publications.