製品の概要

  • 製品名
    Anti-C10orf53 antibody
  • 製品の詳細
    Rabbit polyclonal to C10orf53
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: MPKNAVVILR YGPYSAAGLP VEHHTFRLQG LQAVLAIDGH EVILEKIEDW NVVELMVNEE VIFHCNIKDL EF, corresponding to N terminal amino acids 1-72 of Human C10orf53

  • ポジティブ・コントロール
    • Human testis tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

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  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-C10orf53 antibody (ab122426)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing C10orf53 with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C10orf53 with ab122426. Staining shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.

参考文献

ab122426 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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