製品の概要
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製品名Anti-C10orf118 antibody
C10orf118 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to C10orf118
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アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
antigen, corresponding to internal sequence amino acids 487-618 of Human C10orf118.
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ポジティブ･コントロール
- Human duodenum tissue; RT4 and U251 MG cell lysates, Human liver and tonsil tissue lysates.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab121550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 55088 Human
- SwissProt: Q7Z3E2 Human
- SwissProt: Q8C9S4 Mouse
- Unigene: 159066 Human
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別名
- C10orf118 antibody
- CJ118_HUMAN antibody
- CTCL tumor antigen HD CL 01/L14 2 antibody
see all
Anti-C10orf118 antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-C10orf118 antibody (ab121550)Immunofluorescent staining of Human cell line A-431 shows positivity in golgi. Recommended concentration of ab121550 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-C10orf118 antibody (ab121550)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf118 antibody (ab121550)ab121550, 1/50, staining C10orf118 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells. -
Western blot - Anti-C10orf118 antibody (ab121550)All lanes : Anti-C10orf118 antibody (ab121550) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique
プロトコール
Anti-C10orf118 antibody (ab121550) 使用論文
ab121550 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"