製品の概要

  • 製品名
    Anti-C10orf118 antibody
    C10orf118 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C10orf118
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    antigen, corresponding to internal sequence amino acids 487-618 of Human C10orf118.

  • ポジティブ･コントロール
    • Human duodenum tissue; RT4 and U251 MG cell lysates, Human liver and tonsil tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-C10orf118 antibody (ab121550)
    Immunocytochemistry/ Immunofluorescence - Anti-C10orf118 antibody (ab121550)
    Immunofluorescent staining of Human cell line A-431 shows positivity in golgi. Recommended concentration of ab121550 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Western blot - Anti-C10orf118 antibody (ab121550)
    Western blot - Anti-C10orf118 antibody (ab121550)
    Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf118 antibody (ab121550)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf118 antibody (ab121550)
    ab121550, 1/50, staining C10orf118 in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    Note: strong cytoplasmic positivity in glandular cells.
  • Western blot - Anti-C10orf118 antibody (ab121550)
    Western blot - Anti-C10orf118 antibody (ab121550)
    All lanes : Anti-C10orf118 antibody (ab121550) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique.

参考文献

ab121550 has not yet been referenced specifically in any publications.

ab121550 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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