Anti-c-Rel (phospho S503) 抗体 (ab30624)
Key features and details
- Rabbit polyclonal to c-Rel (phospho S503)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-c-Rel (phospho S503) antibody
c-Rel 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to c-Rel (phospho S503) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human c-Rel (phospho S503).
-
ポジティブ・コントロール
- Breast carcinoma, MDA-MB-435 cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
|
|
IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. -
配列類似性
Contains 1 RHD (Rel-like) domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 5966 Human
- Omim: 164910 Human
- SwissProt: Q04864 Human
- Unigene: 631886 Human
-
別名
- Avian reticuloendotheliosis antibody
- C REL antibody
- C Rel protein antibody
see all
画像
-
All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution
Lane 1 : extracts from
MDA-MB-435 cells (5-30ug).
Lane 2 : extracts from
MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab30624 は 1 報の論文で使用されています。
- Xu X et al. Phosphorylation of NF-?Bp65 drives inflammation-mediated hepatocellular carcinogenesis and is a novel therapeutic target. J Exp Clin Cancer Res 40:253 (2021). PubMed: 34380537