製品の概要

  • 製品名Anti-C Reactive Protein antibody [Y284]
    C Reactive Protein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y284] to C Reactive Protein
  • 特異性ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2.
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.

  • エピトープab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
  • ポジティブ・コントロール
    • WB: Human serum and plasma lysates. IHC-P: Normal human liver and skeletal muscle tissues. IP: Human serum lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.Can be blocked with C Reactive Protein peptide (ab216758).
IP 1/30 - 1/50.
IHC-P 1/100 - 1/250.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • 組織特異性Found in plasma.
    • 配列類似性Belongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    Anti-C Reactive Protein antibody [Y284] 画像

    • All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)

      Lane 1 : Human serum
      Lane 2 : Human plasma

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size : 25 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab32412 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum

      Predicted band size : 25 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

    Anti-C Reactive Protein antibody [Y284] (ab32412) 使用論文

    This product has been referenced in:
    • Garrido M  et al. C-reactive protein expression is up-regulated in apical lesions of endodontic origin in association with interleukin-6. J Endod 41:464-9 (2015). Read more (PubMed: 25748492) »
    • She S  et al. C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma. Int J Oncol 47:543-54 (2015). Read more (PubMed: 26058824) »

    See all 17 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"