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RabMAb

Anti-C Reactive Protein 抗体 [Y284] (ab32412)

製品の概要

  • 製品名
    Anti-C Reactive Protein antibody [Y284]
    C Reactive Protein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y284] to C Reactive Protein
  • 由来種
    Rabbit
  • 特異性
    ab32412 recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human C Reactive Protein aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    (Peptide available as ab216758)

  • エピトープ
    ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
  • ポジティブ・コントロール
    • WB: Human serum and plasma lysates. IHC-P: Normal human liver and skeletal muscle tissues. IP: Human serum lysate.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.Can be blocked with C Reactive Protein peptide (ab216758).
IP 1/30 - 1/50.
IHC-P 1/100 - 1/250.
  • 追加情報
    Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能
      Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
    • 組織特異性
      Found in plasma.
    • 配列類似性
      Belongs to the pentaxin family.
      Contains 1 pentaxin domain.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    画像

    • All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)

      Lane 1 : Human serum
      Lane 2 : Human plasma

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size: 25 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab32412 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum

      Predicted band size: 25 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

    参考文献

    This product has been referenced in:
    • Hanna M  et al. Association between physical activity and the expression of mediators of inflammation in normal breast tissue among premenopausal and postmenopausal women. Cytokine 102:151-160 (2018). Read more (PubMed: 29102166) »
    • Hanna M  et al. Association between local inflammation and breast tissue age-related lobular involution among premenopausal and postmenopausal breast cancer patients. PLoS One 12:e0183579 (2017). Read more (PubMed: 28846716) »

    See all 19 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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