Anti-c-Myc (phospho T58 + S62) 抗体 (ab10568)

製品の概要

  • 製品名Anti-c-Myc (phospho T58 + S62) antibody
    c-Myc 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c-Myc (phospho T58 + S62)
  • 特異性Does not react with nonphosphorylated c-myc. This antibody will recognize c-myc phosphorylated at either T58 or S62, or both T58 and S62.
  • アプリケーション適用あり: Flow Cyt, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH.

  • ポジティブ・コントロール
    • TPA treated A431 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/400. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/500.
WB 1/1000. Predicted molecular weight: 49 kDa.

ターゲット情報

  • 機能Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • 関連疾患Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • 細胞内局在Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Niard antibody
    • Nird antibody
    • Proto-oncogene c-Myc antibody
    • Transcription factor p64 antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc myelocytomatosis viral oncogene homolog antibody
    see all

Anti-c-Myc (phospho T58 + S62) antibody (ab10568) 使用論文

ab10568 has not yet been referenced specifically in any publications.

Product Wall

This antibody will recognize c-myc phosphorylated at either T58 or S62, or both T58 and S62. Thank you for your very good question. I have added this information to the datasheet.

Thank you for your email. Ab10568 recognizes c-myc phosphorylated at phospho-Thr58/Ser62 and does not react with nonphosphorylated c-myc. According to the amino acid sequence (Swiss-Prot database), the predicted band size is around 49 kDa. However, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"