Anti-c-Myc (phospho S62) 抗体 (ab51156)
製品の概要
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製品名Anti-c-Myc (phospho S62) antibody
c-Myc 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to c-Myc (phospho S62)
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由来種Rabbit
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特異性ab51156 detects endogenous levels of Myc only when phosphorylated at serine 62.
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アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-P, IHC-FoFrmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide corresponding to Human c-Myc (N terminal) (phospho S62). The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 62 (P-L-SP-P-S).
Database link: P01106 -
ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human c-Myc protein
- human breast carcinoma tissue, extracts from 293 cells treated with Forskolin, HepG2 cell line Phosphatase inhibitors to increase level of phosphorylation.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, Sodium chloride, 50% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
精製度Protein A purified
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特記事項（精製）The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Corresponding Antibody
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Immunohistochemistry kits
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Positive Controls
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab51156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/100 - 1/500. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). | |
| ELISA | 1/40000. Peptide ELISA. | |
| IHC-P | 1/50 - 1/100. | |
| IHC-FoFr | 1/200. |
ターゲット情報
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機能Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
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関連疾患Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
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翻訳後修飾Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
細胞内局在Nucleus > nucleoplasm. Nucleus > nucleolus.
- Information by UniProt
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参照データベース
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all -
製品の状態c-Myc is also expressed in the cytoplasm.
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別名
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
画像
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Western blot - Anti-c-Myc (phospho S62) antibody (ab51156)All lanes : Anti-c-Myc (phospho S62) antibody (ab51156) at 1/500 dilution
Lane 1 : extracts from 293 cells treated
with 40nM Forskolin for 30min, with phosphopeptide
Lane 2 : extracts from 293 cells treated with 40nM Forskolin for 30min, without phosphopeptide
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody (ab51156)Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left: untreated sample. Right: sample treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (ab51156)Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (ab51156)ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Yan L et al. Downregulation of microRNA-30d promotes cell proliferation and invasion by targeting LRH-1 in colorectal carcinoma. Int J Mol Med 39:1371-1380 (2017). WB ; Human . Read more (PubMed: 28440426) »
- Li J et al. EYA1's Conformation Specificity in Dephosphorylating Phosphothreonine in Myc and Its Activity on Myc Stabilization in Breast Cancer. Mol Cell Biol 37:N/A (2017). Read more (PubMed: 27795300) »
レビューと Q&A
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