Synthetic peptide corresponding to Human c-Myc (N terminal) (phospho S62). The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 62 (P-L-SP-P-S).
Database link: P01106
Our Abpromise guarantee covers the use of ab51156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/100 - 1/500. | |
WB | 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). | |
ELISA | 1/40000. Peptide ELISA. | |
IHC-P | 1/50 - 1/100. | |
IHC-FoFr | 1/200. |
Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left: untreated sample. Right: sample treated with phosphopeptide.
Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.
ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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