Anti-c-Myc 抗体 [9E11] - ChIP Grade (ab56)

製品の概要

  • 製品名
    Anti-c-Myc antibody [9E11] - ChIP Grade
    c-Myc 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9E11] to c-Myc - ChIP Grade
  • アプリケーション
    適用あり: Flow Cyt, ChIP, WB, IP, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Chicken, Human, Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide:

    AEEQKLISEEDL

    conjugated to KLH, corresponding to amino acids 408-420 of Human c-Myc.

  • ポジティブ・コントロール
  • 特記事項

    This antibody clone is manufactured by Abcam.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/200.
ChIP Use at an assay dependent concentration. 2ul per 500ug of extract, see H. Zhang et al.
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa. Additional non-specific bands observed at 75, 110, 140 kDa using mouse and human cells (see Abreview).
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • 関連疾患
    Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾
    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • 細胞内局在
    Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    c-Myc is also expressed in the cytoplasm.
  • 別名
    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

画像

  • c-Myc was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5µg of Mouse monoclonal to c-Myc and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 49KDa; c-Myc

  • Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/1000 dilution + whole cell lysate prepared from huh-7 cells at 15 µg

    Secondary
    Anti-mouse IgG conjugated to HRP at 1/10000 dilution

    Predicted band size : 49 kDa

    Image courtesy of an anonymous Abreview.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa


    Exposure time : 4 minutes

プロトコール

参考文献

This product has been referenced in:
  • Lorentzen A & Mitchelmore C NDRG2 gene copy number is not altered in colorectal carcinoma. World J Clin Oncol 8:67-74 (2017). ChIP ; Human . Read more (PubMed: 28246586) »
  • Gong SJ  et al. Upregulation of PP2Ac predicts poor prognosis and contributes to aggressiveness in hepatocellular carcinoma. Cancer Biol Ther 17:151-62 (2016). Read more (PubMed: 26618405) »

See all 29 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Sample
Human Cell lysate - whole cell (HEK 293)
Specification
HEK 293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
10 µg
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (skin fibroblasts)
Specification
skin fibroblasts
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
NPM1 cMYC binding site
Negative control
IgG pull-down
Username

Abcam user community

Verified customer

投稿 Jul 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
15 µg
Specification
huh-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (mammary epithelial)
Loading amount
80 µg
Specification
mammary epithelial
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell-lines)
Loading amount
20 µg
Specification
Breast cancer cell-lines
Gel Running Conditions
Non-reduced Denaturing (4-20% Nupage gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Cherie Blenkiron

Verified customer

投稿 Jul 18 2008

This antibody was qualified as "ChIP grade" following its application in the following publications; Robert F et al. Global position and recruitment of HATs and HDACs in the yeast genome. Mol Cell 16:199-209 (2004). PubMed: 15494307 Zhang H et al...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Pancreatic cell lines)
Specification
Pancreatic cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Lynda Elghazi-Cras

Verified customer

投稿 Jul 25 2005

Thank you for your enquiry and your interest in our products. I would suggest taking a look at the published references to have some more information about the breast cancer cell types in which c-myc is expressed. Locker AP et al. c-myc oncogen...

Read More

Thsi antibody works well for histochemical localization in paraffin embedded sections. We would recommend a microwave retrieval (10 minutes in 0.01M citrate buffer, pH 6.0, the specific time will depend on the power of the microwave). Leave the 1ary an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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