Anti-c-Myb 抗体 (ab59233)

製品の概要

  • 製品名Anti-c-Myb antibody
    c-Myb 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c-Myb
  • 特異性ab59233 detects endogenous levels of total c-Myb protein.
  • アプリケーション適用あり: ICC/IF, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human c-Myb around the phosphorylation site of serine 12 (Y-S-SP-D-E).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab59233 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
  • 配列類似性Contains 3 HTH myb-type DNA-binding domains.
  • ドメインComprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.
  • 翻訳後修飾Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C myb antibody
    • c myb protein (140 AA) antibody
    • c-myb protein (140 AA) antibody
    • c-myb_CDS antibody
    • c-myb10A_CDS antibody
    • c-myb13A_CDS antibody
    • c-myb14A_CDS antibody
    • c-myb8B_CDS antibody
    • Cmyb antibody
    • efg antibody
    • Myb antibody
    • MYB_HUMAN antibody
    • ONCOGENE AMV antibody
    • Oncogene MYB antibody
    • Proto oncogene c Myb antibody
    • Proto-oncogene c-Myb antibody
    • Transcriptional activator Myb antibody
    • v myb avian myeloblastosis viral oncogene homolog antibody
    • v-myb avian myeloblastosis viral oncogene homolog antibody
    see all

Anti-c-Myb antibody 画像

  • Staining of C-Myb in human breast carcinoma tissue (paraffin section) with ab59233 (1/50 dilution) by Immunohistochemistry; in the absence or presence of the immunizing peptide.
  • ICC/IF image of ab59233 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59233, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-c-Myb antibody (ab59233) 使用論文

ab59233 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Sample Human Tissue sections (Lymph node)
Specification Lymph node
Permeabilization No
Fixative Acetone
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投稿 Jul 15 2013

Thank you for your enquiry. I am very pleased to hear the customer would like to make use of our AbTrial offer and test ab59233 in IHC-Fr.


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