製品の概要
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製品名Anti-c-Kit (phospho Y703) antibody
c-Kit 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to c-Kit (phospho Y703)
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由来種Rabbit
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特異性ab62154 detects endogenous levels of c-Kit only when phosphorylated at tyrosine 703 (Human: Tyr703; Mouse: Tyr702).
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アプリケーション適用あり: WB, IHC-P, ELISAmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic phosphopeptide derived from human c-Kit around the phosphorylation site of tyrosine 703 (A-L-YP-K-N).
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ポジティブ･コントロール
- Human breast carcinoma tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab62154 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab62154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/100. | |
| ELISA | 1/5000. |
ターゲット情報
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機能Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
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組織特異性Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
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関連疾患Piebald trait
Gastrointestinal stromal tumor
Testicular germ cell tumor
Leukemia, acute myelogenous -
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7. -
細胞内局在Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
- Information by UniProt
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参照データベース
- Entrez Gene: 3815 Human
- Entrez Gene: 16590 Mouse
- Omim: 164920 Human
- SwissProt: P10721 Human
- SwissProt: P05532 Mouse
- Unigene: 479754 Human
- Unigene: 247073 Mouse
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別名
- C Kit antibody
- c-Kit antibody
- c-Kit Ligand antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Kit (phospho Y703) antibody (ab62154)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Kit (phospho Y703) antibody (ab62154) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide. -
Western blot - c-Kit (phospho Y703) antibody (ab62154)This image is courtesy of an Abreview submitted by Antibody SolutionsAll lanes : Anti-c-Kit (phospho Y703) antibody (ab62154)
Lane 1 : Colon cancer lysate (human)
Lane 2 : U2OS lysate (human)
Lane 3 : Renal cancer cell line (human)
Secondary
All lanes : Swine ati-rabbit HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 6 minutes
参考文献
This product has been referenced in:
- Li G et al. Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation. Exp Ther Med 13:1093-1100 (2017). WB . Read more (PubMed: 28450948) »
- Grewal N et al. Histopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy? Heart Vessels 31:795-806 (2016). Read more (PubMed: 26129868) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"