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Our Abpromise guarantee covers the use of ab25022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab18542-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|Functional Studies||Use at an assay dependent concentration. Can be used for cell homing studies, blockade of hematopoiesis and disruption fo melanocyte development.|
|ICC/IF||Use at an assay dependent concentration.|
Flow Cytometry analysis of C57BL/6 mouse bone marrow cells labeling c-Kit with ab25022 at 0.3 μg/106 cells dilution. Rat Anti-Mouse CD45R-PE followed by Streptavidin-FITC was used as the secondary antibody.