Anti-c-Jun (phospho T93) 抗体 [EPR2237] (ab81319)
製品の概要
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製品名Anti-c-Jun (phospho T93) antibody [EPR2237]
c-Jun 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2237] to c-Jun (phospho T93)
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由来種Rabbit
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特異性The antibody only detects c-Jun when phosphorylated at Threonine 93.
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アプリケーション適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human c-Jun aa 50-150 (phospho T93).
Database link: P05412 -
ポジティブ･コントロール
- NIH/3T3 cell lysates treated with Anisomycin
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特記事項
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2237
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アイソタイプIgG
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab81319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). | |
| IP | 1/50. |
ターゲット情報
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機能Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
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配列類似性Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻訳後修飾Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 3725 Human
- Entrez Gene: 16476 Mouse
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P05627 Mouse
- Unigene: 696684 Human
- Unigene: 275071 Mouse
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別名
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
画像
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![Western blot - c-Jun (phospho T93) antibody [EPR2237] (ab81319)](http://a.abcam.com/ps/datasheet/images/81/ab81319/c-Jun-Primary-antibodies-ab81319-1.jpg)
Western blot - c-Jun (phospho T93) antibody [EPR2237] (ab81319)All lanes : Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319) at 1/5000 dilution
Lane 1 : NIH/3T3
cell lysates untreated
Lane 2 : NIH/3T3
cell lysates treated
with Anisomycin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
参考文献
ab81319 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"