Anti-c-Jun (phospho T93) 抗体 [EPR2237] (ab81319)

製品の概要

• 製品名
  Anti-c-Jun (phospho T93) antibody [EPR2237]
  c-Jun 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EPR2237] to c-Jun (phospho T93)
• 特異性
  The antibody only detects c-Jun when phosphorylated at Threonine 93.
• アプリケーション
  適用あり: WB, IPmore details
  適用なし: Flow Cyt,ICC/IF or IHC-P
• 種交差性
  交差種: Mouse, Human
  
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human c-Jun aa 50-150 (phospho T93).
  Database link: P05412

• ポジティブ･コントロール
  • NIH/3T3 cell lysates treated with Anisomycin
• 特記事項

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

  Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EPR2237
• アイソタイプ
  IgG
• 研究分野
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • SMADs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Immunology
  • Innate Immunity
  • TLR Signaling

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Phosphoprotein ELISA kits

アプリケーション

ターゲット情報

• 機能
  Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
• 配列類似性
  Belongs to the bZIP family. Jun subfamily.
  Contains 1 bZIP (basic-leucine zipper) domain.
• 翻訳後修飾
  Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
  Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
  Acetylated at Lys-271 by EP300.
• 細胞内局在
  Nucleus.

• Target information above from: UniProt accession P05412 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 3725 Human
  • Entrez Gene: 16476 Mouse
  • Omim: 165160 Human
  • SwissProt: P05412 Human
  • SwissProt: P05627 Mouse
  • Unigene: 696684 Human
  • Unigene: 275071 Mouse
• 別名
  • Activator protein 1 antibody
  • AP 1 antibody
  • AP1 antibody

  • cJun antibody
  • Enhancer Binding Protein AP1 antibody
  • Jun Activation Domain Binding Protein antibody
  • JUN antibody
  • Jun oncogene antibody
  • JUN protein antibody
  • Jun proto oncogene antibody
  • JUN_HUMAN antibody
  • JUNC antibody
  • Oncogene JUN antibody
  • p39 antibody
  • Proto oncogene c jun antibody
  • Proto oncogene cJun antibody
  • Proto-oncogene c-jun antibody
  • Transcription Factor AP 1 antibody
  • Transcription factor AP-1 antibody
  • Transcription Factor AP1 antibody
  • V jun avian sarcoma virus 17 oncogene homolog antibody
  • V jun sarcoma virus 17 oncogene homolog (avian) antibody
  • V jun sarcoma virus 17 oncogene homolog antibody
  • V-jun avian sarcoma virus 17 oncogene homolog antibody
  • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody

  see all

プロトコール

• Western blot protocols
• Immunoprecipitation protocols