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RabMAb

Anti-c-Jun 抗体 [EP693Y] (ab40766)

製品の概要

  • 製品名
    Anti-c-Jun antibody [EP693Y]
    c-Jun 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP693Y] to c-Jun
  • 由来種
    Rabbit
  • 特異性
    PBS only lot tested.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human c-Jun aa 300-400 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: PC-12 and NIH/3T3 whole cell lysate. HEK-293 cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/50.

ターゲット情報

  • 機能
    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • 配列類似性
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻訳後修飾
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

画像

  • All lanes : Anti-c-Jun antibody [EP693Y] (ab40766) at 0.212 µg/ml

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

    Exposure time: Lane 1- 41 seconds
                             Lane 2 - 1 second


  • Anti-c-Jun antibody [EP693Y] (ab40766) at 1/5000 dilution + 10ug 293 cell lysate

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • Tissue: Paraffin-embedded human colon carcinoma.
    Staining: ab40766 at 1/100 dilution
  • Immunofluorescent staining of HeLa cells
    ab40766 at 1/100 dilution

参考文献

This product has been referenced in:
  • Syc-Mazurek SB  et al. JUN is important for ocular hypertension-induced retinal ganglion cell degeneration. Cell Death Dis 8:e2945 (2017). IHC ; Mouse . Read more (PubMed: 28726785) »
  • Mac Nair CE  et al. Tumor necrosis factor alpha has an early protective effect on retinal ganglion cells after optic nerve crush. J Neuroinflammation 11:194 (2014). Read more (PubMed: 25407441) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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