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Synthetic peptide corresponding to Human c-Jun aa 222-251 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P05412
Our Abpromise guarantee covers the use of ab86740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC - Wholemount||1/100.|
|WB||1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
ab86740 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab86740 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.
ab86740 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"