Anti-c-Jun 抗体 (ab31417)

製品の概要

  • 製品名Anti-c-Jun antibody
    c-Jun 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c-Jun
  • 特異性This antibody detects endogenous levels of c-Jun protein around Tyrosine 170.
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, ELISA, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from Human c-Jun around the phosphorylation site of Tyrosine 170.

  • ポジティブ・コントロール
    • Extracts of Hela cells and breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • 配列類似性Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • 翻訳後修飾Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun antibody 画像

  • ab31417 staining human c-Jun in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

  • All lanes : Anti-c-Jun antibody (ab31417) at 1/500 dilution

    Lane 1 : Extracts of Hela cells.
    Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.


    Predicted band size : 36 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab31417 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31417, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Anti-c-Jun antibody (ab31417) 使用論文

ab31417 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - used for EMSA
Sample Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
Specification Renal cancer cell lines - nuclear extract
Total protein in input 3 µg
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Verified customer

投稿 Mar 18 2014

I apologize for the delay in replying to your question. Ab8754 recognizes non-phosphorylated proteins. As for the other two antibodies, ab31417 and ab31419, here is the information I obtained from the originator: We did WB again on these two antibod...

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