製品の概要

  • 製品名Anti-c-Fos (phospho T325) antibody
    c-Fos 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c-Fos (phospho T325)
  • 特異性ab27793 recognises the cFos phosphorilated at threonine 325 form.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from the region of human cFos that contains threonine 325.

  • ポジティブ・コントロール
    • Human epidermoid carcinoma (A431) cells stimulated with EGF or 15% serum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent dilution. PubMed: 20410304

ターゲット情報

  • 機能Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
  • 配列類似性Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.
  • 翻訳後修飾Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator protein 1 antibody
    • AP 1 antibody
    • C FOS antibody
    • Cellular oncogene c fos antibody
    • Cellular oncogene fos antibody
    • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
    • FBJ murine osteosarcoma viral oncogene homolog antibody
    • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
    • FBJ Osteosarcoma Virus antibody
    • FOS antibody
    • FOS protein antibody
    • FOS_HUMAN antibody
    • G0 G1 switch regulatory protein 7 antibody
    • G0/G1 switch regulatory protein 7 antibody
    • G0S7 antibody
    • Oncogene FOS antibody
    • p55 antibody
    • proto oncogene c Fos antibody
    • Proto oncogene protein c fos antibody
    • Proto-oncogene c-Fos antibody
    • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
    see all

Anti-c-Fos (phospho T325) antibody 画像

  • All lanes : Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution (diluted in a 3% Milk TBST buffer.)

    Lane 1 : Non EGF treated A431 cell lysate
    Lane 2 : EGF treated A431 cell lysate
    Lane 3 : EGF treated A431 cell lysate treated with Lambda phosphatase

    Secondary
    goat F(ab’)2 anti rabbit IgG HRP conjugate

    Predicted band size : 41 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

Anti-c-Fos (phospho T325) antibody (ab27793) 使用論文

This product has been referenced in:
  • Nakakuki T  et al. Ligand-specific c-Fos expression emerges from the spatiotemporal control of ErbB network dynamics. Cell 141:884-96 (2010). WB ; Human . Read more (PubMed: 20493519) »
  • Asim M  et al. Helicobacter pylori induces ERK-dependent formation of a phospho-c-Fos c-Jun activator protein-1 complex that causes apoptosis in macrophages. J Biol Chem 285:20343-57 (2010). WB, IP ; Mouse . Read more (PubMed: 20410304) »

See all 3 Publications for this product

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I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and would like to make some suggestions to improve...

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Thank you for your enquiry. Ab27793 has not yet been tested for application in IF (only Western blotting so far)but ab17933 - Rabbit polyclonal to c-Fos (phospho T232) - has been tested in IF. There is an image on the online datasheet with more more in...

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